Day Milk vs Night Milk - What’s the Difference?

Breast milk is dynamic and always changing. It offers complete nutrition to your baby, and hydration and lays the foundation for their immune system. Packed with vitamins, minerals, hormones, live cells, healthy bacteria (probiotics), and food for our good gut bacteria(prebiotics), it gives the baby everything they need for the first 6 months of their life to grow their brain and body.

Breast milk changes during the different stages of lactation, from feeding to feeding and from day to night. It responds to information from the baby and mother and adjusts to best protect the baby from illness and fight off unwanted germs.

Baby’s biological clock

Babies are not born with a circadian rhythm of day and night. They get lots of information from their environment as well as the mother’s milk over time to develop their internal clock. Babies' sleep cycle matures and is a developmental milestone, not something we need to teach, nor can teach. It will happen when they are ready and over time.

Exposure to light and dark help inform you and your baby’s internal clock. We are hardwired to get sleepy when the sun goes down and more alert when the sun is up. Artificial light can interfere with our natural rhythms. Try keeping light low and limiting light exposure during the night and get plenty of sunlight with your baby during the day.

In breastmilk

• The hormone cortisol is 3 times higher in your milk in the morning than it is late in the day. Cortisol is the hormone that is released during stress but also aids in wakefulness. A baby doesn’t begin to have a cortisol circadian rhythm until about 8 weeks old.(1) 
• Melatonin peaks in the evening. Melatonin makes the baby more sleepy. It increases when it is dark. Keeping lights dim or dark during the night means your milk continues to make milk higher in melatonin.(3)
• Adenosine, guanosine, and uridine are 3 nucleotides in breastmilk that are highest in breastmilk between 8 pm - 8 am. They calm the nervous system, helping the baby to relax and fall asleep.(2)

Milk supply

Prolactin is the hormone that signals more milk to be made. It begins to rise after the baby begins to suck. As milk is emptied from the breast, the pituitary gland releases prolactin which tells the cells in the mammary gland to begin to draw nutrients from the blood to synthesize milk and store it for when the baby feeds again. The more often the baby nurses, the more often this signaling takes place and the more often milk is made. 

Prolactin levels have their own circadian rhythm and are highest in the middle of the night, peaking between 2-5 am. When milk is removed in the middle of the night when prolactin is already elevated, it is very protective of mom’s overall milk supply. When prolactin levels are raised from the baby nursing regularly including during the night, the more likely you are to continue to experience lactational amenorrhea.(4)

In the late afternoon and early evening hours when the baby cluster feeds, the milk-making hormone, prolactin, is at its lowest. Your breasts are naturally less full and the baby will ask to feed more frequently. Older babies are busy discovering their world and likely distracted when they nurse so only nurse for shorter periods, will make up for the time missed nursing during the nighttime further protecting mom’s milk supply. In fact, babies get about 20% of their calories from milk they drink during the night.

Fat content

Human milk has relatively low-fat content but makes up about half the calories your baby takes.(5) It doesn't have a circadian rhythm, yet is higher during the early evening hours vs early morning. When the breasts are less full, the fat content in milk is higher.(6) Going longer stretches between feeding or pumping, the breast will have more water content when the baby begins to nurse before more fat is mixed into the milk. You might notice when you feel less full of milk during periods of cluster feeding. Your baby gets more fat in the milk during those feeds and they have a longer stretch of sleep afterward. Fat content in milk is highest between 12-6 pm.(7)

Immune factors, vitamins, and minerals

During the daytime is when antibodies and white blood cells appear to be highest in breast milk. Cytokines are a group of proteins that control the immune response and are highest in milk during the day.(8) This means there is a lot of communication being passed between mom and baby through her milk protecting the baby from illness.

Milk in the morning has higher magnesium, zinc, potassium, and sodium while nighttime milk is higher in Vitamin E. Iron is highest at noon.(9)

Feeding on cue for bottle feeding too

Get to know the cues of when your baby is ready to eat and offer before they show late feeding cues, especially crying. A baby crying when they are hungry is equivalent to when you wait too long to eat and become “hangry”. You know that more frantic, kind of annoyed angry feeling you get and you just start grabbing whatever is in sight to eat rather than choosing your meal wisely, sitting down and eating it while relaxed, taking time to chew your food and eat more slowly. When your baby is crying because they are hungry, they have a harder time coordinating their feeding, taking in more air, or have less comfortable digestion.

When you follow your baby’s cues and practice side-lying bottle feeding which is a style of bottle feeding more baby controlled than parent-led and a slower way to feed, they have the opportunity to pay attention to their satiety. Some feeds may be smaller like 1.5-2 oz while other feeds will be 3-4 oz. They will not be on a set schedule, but able to follow their natural hunger so times between feedings will vary as well.

By following their cues, you allow them to follow their natural biological rhythm and know that they are getting just what they need when they need it from your milk.

Feeding pumped breastmilk

The more we learn about the rhythms of all the components of breast milk, the more we may want to consider following mother nature’s lead. If you pump milk for your baby, it is recommended to label your milk with the time of day or night that it was pumped. 

For nighttime bottles, give your baby milk that has been pumped at night and the same for during the day. In this way, your baby naturally gets milk to keep their body in sync the same as if they were directly feeding at the breast/chest. Milk pumped during the day will deliver the ingredients to your baby that stimulate activity and the milk pumped at night, if given at night, will help them relax and drift off to sleep.

Footnotes:

1. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6175794/
2. https://www.sciencedaily.com/releases/2009/10/091001091757.htm
3. https://www.llli.org/breast-milks-circadian-rhythms-2/
4. https://pubmed.ncbi.nlm.nih.gov/2715293/
5. https://www.mdpi.com/2072-6643/8/5/279
6. https://pubmed.ncbi.nlm.nih.gov/8311942/
7. https://internationalbreastfeedingjournal.biomedcentral.com/articles/10.1186/s13006-020-00291-y
8. https://www.tandfonline.com/doi/full/10.1080/09291016.2015.1056434
9. https://dornsife.usc.edu/news/stories/3060/breast-milk-helps-babies-tell-circadian-rhythm-from-mom/#:~:text=For%20example%2C%20levels%20of%20cortisol,milk%20than%20in%20evening%20milk.

Other Resources:

https://www.e-cep.org/upload/pdf/cep-2020-00059.pdf

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7468880/